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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Hsa_circ_0005273 facilitates breast cancer tumorigenesis by regulating YAP1-hippo signaling pathway

Fig. 3

Hsa_circ_0005273 serves as a sponge for miR-200a-3p. A Venn diagram showing the potential target miRNAs of hsa_circ_0005273. B-D Expression of miRNAs in BC cells transfected with si-circ_0005273 or LV-circ_0005273. E RIP experiments were performed in HEK293T cells, and the co-precipitated RNA was subjected to RT-qPCR for hsa_circ_0005273 and miR-200a-3p. F MiR-200a-3p was pulled down and enriched with hsa_circ_0005273 specific probe and then detected by RT-qPCR. G Putative complementary sites within miR-200a-3p and hsa_circ_0005273 predicted by bioinformatics analysis (starbase). H Dual luciferase reporter assays demonstrated that miR-200a-3p is a direct target of hsa_circ_0005273. I Detection of colocalization of hsa_circ_0005273 and miR-200a-3p in cytoplasm by RNA FISH assay (magnification, × 400). Green, miR-200a-3p; Red, hsa_circ_0005273; Blue, DAPI. *p < 0.05, **p < 0.01,*** p < 0.001,**** p < 0.0001

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