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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Neuropilin 1 and Neuropilin 2 gene invalidation or pharmacological inhibition reveals their relevance for the treatment of metastatic renal cell carcinoma

Fig. 1

NRP1 or NRP2 gene invalidation results in inhibition of cell proliferation and migration. a The locus of the NRP1 gene was sequenced in control (NRP1 Ctrl) and in two independent clones (#NRP1 2.2 and #NRP1 2.7) KO for NRP1. b The locus of NRP2 was sequenced in control (NRP2 Ctrl) and in two independent clones (#NRP2 2.3 and #NRP1 2.28) KO for NRP2. c NRP1 and NRP2 mRNA levels were tested by qPCR in control (786O), in two independent clones (#NRP1 2.2 and 2.7) KO for NRP1, and in two independent clones (#NRP2 2.3 and 2.28) KO for NRP2. d NRP1 and NRP2 protein levels were evaluated by flow cytometry in control (786O), in two independent clones (#NRP1 2.2 and 2.7) KO for NRP1, and in two independent clones (#NRP2 2.3 and 2.28) KO for NRP2. e The proliferation of NRP1 and NRP2 KO cells were tested by counting the cells at the indicated time points. f The migration of NRPs KO cells was determined in scratch assays by measuring the time of wound closure. g VEGFA and VEGFC expression was tested in control (Ctrl) and KO clones by ELISA. *p < 0.05; **p < 0.01; *** p < 0.001

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