Fig. 1

Identification of circ_0119872 in uveal melanoma (UM). a Top 10 significantly down-regulated and up-regulated circRNAs in UM tissues ranked by FC. b Schematic diagrams show that circ_0119872 is composed of exons 4 and 5 of RASGRP3. The existence of circ_0119872 was demonstrated by PCR, and its back-splicing junction was verified by Sanger sequencing. c PCR assay with divergent and convergent primers indicating that circ_0119872 is present in the OCM-1A and MUM-2B cell lines. GAPDH was used as a negative control. d The expression of and RASGRP3 after RNase R treatment in OCM-1A and MUM-2B cells. e The expression level of circ_0119872 in a human retinal pigment epithelial cell line (APRE-19) and UM cell lines (SP6.5, VUP, OCM-1, 92–1, OCM-1A, MUM-2B, OM431). f The expression level of circ_0119872 in 10 pairs of UM and adjacent non-tumour tissues. g and h RNA-FISH and RNA nucleus/cytoplasm separation assay indicates the location of circ_0119872. Nuclei are stained blue with DAPI. Circ_0119872 is stained green with FAM. Bar graphs show the statistical analysis of three independent experiments (* P < 0.05)