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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Circ_0119872 promotes uveal melanoma development by regulating the miR-622/G3BP1 axis and downstream signalling pathways

Fig. 3

Circ_0119872 interacts with miR-622 in UM cells. a Schematic diagram displaying the probe specifically designed for circ_0119872. b and c Gel electrophoresis and qRT-PCR results showed that the circ_0119872 probe could specifically pull down the circular form of circ_0119872. d and e The relative expression levels of ten miRNA candidates pulled down by the circ_0119872 probe were detected by qRT-PCR. f Circ_0119872 was enriched by biotinylated wild-type miR-622 or its mutant, and qRT-PCR was used to determine the relative circ_0119872 levels. g RNA-FISH assay showed the colocalization between circ_0119872 and miR-622 in UM cells. MiR-622 probes were labelled with Cy3. Circ_0119872 probes were labelled with FAM. Nuclei were stained with DAPI. Data are represented as the mean ± SD of three independent experiments (* P < 0.05)

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