Fig. 5

MiR-622 directly targets the G3BP1 3’UTR. a The Venn diagram shows that G3BP1 is commonly predicted by TargetScan, miRDB and miRTarBase. b Schematic diagram of two potential binding sites of miR-622 and the G3BP1 3’UTR. c Dual-luciferase reporter assay shows that miR-622 directly targets the G3BP1 3’UTR by binding to site #2. d The protein levels of G3BP1 detected by western blotting in miR-622-overexpressing and knockdown cells. e and f Circ_0119872 and miR-622 can co-regulate the protein level of G3BP1. g G3BP1 expression in the GSE44295 dataset. h and i Progression-free survival and disease-specific survival in the TCGA UVM dataset with high versus low levels of G3BP1 mRNA. J. G3BP1 protein expression in 10 UM tissues (T) and paired adjacent non-tumorous tissues (N). Bar graphs show the statistical analysis of three independent experiments (* P < 0.05)