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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Hypoxic tumour cell-derived exosomal miR-340-5p promotes radioresistance of oesophageal squamous cell carcinoma via KLF10

Fig. 4

KLF10 is a direct target for miR-340-5p. a Illustration of the putative predicted miR-340-5p binding site in the KLF10 mRNA 3′-UTR. b-c Expression of KLF10 in OSCC cells transfected with relevant lentiviral vectors was detected by qRT-PCR (b) and Western blotting (c). d Expression of KLF10 in OSCC cells was decreased after cocultured with hypoxic EVs. e Expression of KLF10 was detected by IHC in xenografts transfected with the relevant lentiviral vectors (scale bar = 200 μm). f Luciferase reporter assays were conducted to verify that miR-340-5p binds to the 3′-UTR region of KLF10 directly. g RIP assays confirmed the binding status between miR-340-5p and KLF10 in treated and untreated OSCC cells. NC: cells transfected with negative control lentiviral vectors; miR-340-5p: cells transfected with miR-340-5p mimic lentiviral vectors

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