Fig. 5

miR-340-5p induces radioresistance by affecting KLF10. a KLF10 knockdown attenuated apoptosis caused by irradiation in OSCC cells. b KLF10 knockdown decreased the expression of the DNA damage repair protein γ-H2AX (scale bar = 20 μm). c Western blot analysis was used to detect the expression of several key factors involved in apoptosis and DNA damage repair in OSCC cells transfected with KLF10 knockdown and control lentiviral vectors. d-f Overexpression of KLF10 in OSCC cells transfected with the miR-340-5p mimic lentiviral vectors rescued the apoptosis (d), DNA damage repair (e, scale bar = 20 μm), and the expression of several key proteins after irradiation (f). NC: cells transfected with negative control lentiviral vectors; sh-KLF10: cells transfected with KLF10 knockdown lentiviral vectors; miR-340-5p: cells transfected with miR-340-5p mimic lentiviral vectors; miR-340-5p + KLF10: cells cotransfected with miR-340-5p mimic and KLF10 mimic lentiviral vectors