Fig. 2

Characteristics and detection methods of 5’tiRNA-His-GTG. a tsRNAs are mainly divided into five types: i-tRF, 3′-tRF, 5′-tRF, 3′-tiRNA, and 5′-tiRNA. b 5’tiRNA-His-GTG was derived from mature tRNA-His-GTG with a length of 35 nt. c 5’tiRNA-His-GTG is mainly located in the cytoplasm, as assessed using a nuclear-cytoplasmic fractionation assay. d Schematic diagram of the two methods for detecting 5’tiRNA-His-GTG. e Relative expression level of 5’tiRNA-His-GTG with and without RNA pre-treatment using the Stem-loop RT method. f The product of qRT-PCR was confirmed by Sanger Sequencing. All data are representative of at least three independent experiments and are presented as the means ± SD. tsRNA, tRNA-derived small RNA; tRF, tRNA-derived fragment; tiRNA, tRNA-derived and stress-induced small RNA; qRT-PCR, quantitative real-time reverse transcription PCR