Fig. 3

5’tiRNA-His-GTG plays an oncogenic role in CRC. a 5’tiRNA-His-GTG endogenous expression in CRC cell lines, as assessed using qRT-PCR. b The 5’tiRNA-His-GTG mimic promoted cell reproductive capacity, while the 5’tiRNA-His-GTG inhibitor significantly reduced cell proliferation of HCT116, LoVo, and RKO cells, as assessed using CCK8 assays. c 5’tiRNA-His-GTG inhibition reduced anchorage-dependent growth. d 5’tiRNA-His-GTG inhibition induced apoptosis, as assessed using FACS analysis. e Western blotting analysis showing the levels of apoptosis indicators (PARP, Cleaved-PARP, Caspase9, Cleaved-Caspase9) in 5’tiRNA-His-GTG inhibitor-treated HCT116 and LoVo cells. f At the end of the xenograft experiment, tumors from the five groups were dissected and photographed. g Tumor growth summarized using a line chart. h Tumor weights are shown in a histogram. i Representative TUNEL assay results. Apoptotic cells have brown-stained nuclei (the DNase I group is the positive control). *p < 0.05, **p < 0.01. All data are representative of at least three independent experiments and are presented as the means ± SD. CRC, colorectal cancer; qRT-PCR, quantitative real-time reverse transcription PCR; CCK8, cell counting kit 8; FACS, fluorescence activated cell sorting; PARP, poly (ADP-Ribose) polymerase 1; TUNEL, terminal deoxynulceotidyl transferase nick-end-labeling