Fig. 6

Attenuation of LATS2 partly rescues 5’tiRNA-His-GTG inhibition-mediated suppressive effects on CRC cells. a CCK8 assay showing inhibitor group increased cell proliferation in the Si-LATS2 / 5’tiRNA-His-GTG in HCT116 and LoVo cells, compared with that in the Si-NC / 5’tiRNA-His-GTG inhibitor group. b Colony formation assay showing that knockdown of LATS2 weakened the repressive effect of the 5’tiRNA-His-GTG inhibitor on colony formation. c FACS analysis showing that knockdown of LATS2 reduced the cell apoptosis induced by the 5’tiRNA-His-GTG inhibitor. d Western blotting of LATS2, phospho-YAP (Ser127), and YAP expression upon Si-LATS2 or/and 5’tiRNA-His-GTG inhibitor transfection in the indicated CRC cells. e Some downstream genes of the hippo signaling pathway are upregulated, as measured using qRT-PCR. f Schematic diagram showing that hypoxia-induced 5’tiRNA-His-GTG mediates the expression of LATS2, thus contributing to the development of CRC. *p < 0.05, **p < 0.01, ns: not significant. All data are representative of at least three independent experiments and are presented as the means ± SD. LATS2, large tumor suppressor kinase 2; CRC, colorectal cancer; CCK8, cell counting kit 8; FACS, fluorescence activated cell sorting; YAP, Yes-associated protein