Fig. 2

Moderate doses of GKT771 treatment do not induce HCC cell cytotoxicity in vitro. To determine the concentration-dependent cytotoxicity of specific NOX1 inhibition, human Huh7 a-c and Hep3B d-f and murine Hepa1-6 g-i HCC cells were treated for 24 hours with 1.25–100 µM GKT771 (NOXi), vehicle (V), vehicle-free medium (M) or positive controls (S and Tx). MTT (mitochondrial metabolism), LDH (membrane integrity, necrosis) and CaspGlo® (apoptosis) assays were performed. The results are shown as percentages of the vehicle-treated control group. S: Staurosporin; Tx: Triton-X; M: Vehicle-free medium; V: Vehicle. Data shown as mean ± SD, ^nsnot significant; ^*p < 0,05; ^**p < 0,01; ^***p < 0,001; ^****p < 0,0001 compared to vehicle, unless indicated differently