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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: NOX1 inhibition attenuates the development of a pro‐tumorigenic environment in experimental hepatocellular carcinoma

Fig. 3

Cytotoxic effects of NOX1 inhibition on mouse BMDMs and human monocyte cell line THP1. Murine bone marrow-derived single cell isolates and THP1 cells were cultured in medium containing M-CSF or PMA respectively, to induce differentiation into macrophages. The resulting BMDMs and THP1 cells were treated with vehicle/100 µM of GKT771 for 48 h with and without LPS-stimulation (1 µg/ml). a-b MTT and LDH analyses were performed to determine the mitochondrial metabolic state and the disruption of cellular membrane integrity respectively. NOX1 inhibition attenuates LPS-induced BMDM polarization. c-d mRNA expression of different inflammatory markers was examined via RT-qPCR. Data presented as relative expression to vehicle-treated controls in the absence of LPS. nsnot significant; * p < 0,05; ** p < 0,01; *** p < 0,001; **** p < 0,0001 compared to vehicle without LPS, unless indicated differently

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