Fig. 8

NOX1 inhibition attenuates the induction of the pro-inflammatory microenvironment in established HCC in mice. Mice received weekly DEN (35 mg/kg) injections for 25 or 20 weeks and were administered twice or single daily doses of vehicle/GKT771 via oral gavage in an early or delayed treatment regimen, respectively. Healthy livers, HCC lesions and surrounding tissue was assessed (a) RT-qPCR analysis to determine the mRNA expression levels of inflammatory (IL6, TNFα, CCL2, CCR2) and inflammasome (NLRP3, caspase 1, IL1β, iNOS) markers and the immune suppressive marker PDL1. b Multiplex analysis of IL6, TNFα, CCL2 levels and ROS levels (µM H202 per µg protein). ^nsnot significant; * p < 0,05; ** p < 0,01; *** p < 0,001; **** p < 0,0001 compared to vehicle-treated healthy controls, unless indicated differently