Fig. 1

miR-155 depletion in murine mammary tumors results in disrupted PGE2/PGD2 balance and abnormal expressions of related metabolic enzymes. a PGE2/PGD2 measurement from two sets of mammary tumors with stable knockdown of miR-155 (marked as Lav670 + miRZIP155 and Lav714 + miRZIP155), showing significantly reduced PGE2/PGD2 ratio (n = 20). b-c Two graphs showing PGE2(b) or PGD2(c) level, from the plasma samples of miR-155 ^KO/KO or miR-155^KO/+ mouse with mammary tumors (n = 10). d-g Relative RNA expression of Ptges1(d), Ptges2(e), Ptgds(f) and 15-Pgdh(g) analyzed in miR-155 ^KO/KO or miR-155^KO/+ mammary tumors (n = 10). h Western blot analysis of key metabolic enzymes of PGE2/PGD2 production in miR-155 ^KO/KO and miR-155^KO/+ mammary tumors (n = 10). COX1, COX2, PTGES1, PTGES2, PTGDS, and 15-PGDH expressions are shown. β-actin was probed to ensure equal loading