Fig. 2
Antitumor therapeutic effect of the anti-16E6 scFvI7nuc and anti-16E7 scFv43M2SD on higher inoculum of TC-1 cells. C57/BL6 mice were injected subcutaneously with 2 × 105 TC-1 cells and treated intratumor with plasmids expressing the anti-16E6 scFvI7nuc and anti-16E7 scFv43M2SD or irrelevant scFvs (CTR scFvs). Each line represents a different mouse within the same treatment group. Two types of control mice treated with R4nuc and R4sec were combined into a single group consisting of 4 total mice to keep the number of mice to a minimum. Treatment was performed four times at one-week intervals, in parallel with tumor size monitoring by caliper measurement. T is the time point in weeks after tumor cell challenge. Tumor growth is expressed as the tumor volume in mm3 at the indicated time points. Significant p values with respect to the corresponding controls are reported on the graphs. In the histogram, the mean weight ± SD of the tumors treated with therapeutic scFvs, and their controls, excised after mice sacrifice, is reported. Differences are significant with p = 0.004 for scFvI7nuc and p = 0.006 for scFv43M2SD