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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Intrabodies targeting human papillomavirus 16 E6 and E7 oncoproteins for therapy of established HPV-associated tumors

Fig. 4

Treatment with anti-16E6 and -16E7 scFvs induces cell death by apoptosis in tumor mass. H&E staining (panels a) and active caspase-3 (cleaved caspase) staining (panels b) of HPV tumor sections from mice treated with scFvI7nuc (I7nuc), scFv43M2SD (43M2SD) and the irrelevant scFvR4nuc (R4nuc) and scFvR4sec (R4sec) are presented. Tumors from one representative mouse for each treatment group are shown. Referring to the numbering shown in Fig. s1, mouse # 3501 for I7nuc, #3504 for R4nuc, #3520 for M2SD and #3514 for R4sec were used. With H&E staining, wide areas of necrosis are visible in tumors treated with both therapeutic scFvs, while rare signs of necrosis are detected in tumors treated with the control scFvs (a). With active caspase-3 staining, brown-stained areas meaningful of apoptotic cell death are clearly visible in tumors treated with both therapeutic scFvs while rare apoptotic areas are detected in tumors treated with the control scFvs (b)

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