Fig. 5
From: Targeting dopamine receptor D2 as a novel therapeutic strategy in endometrial cancer
ONC201 reduced adhesion and invasion in EC cells. The ECC-1 and KLE cells were cultured with different doses of ONC201 for 90 min. Adhesion was determined by laminin-1 assay (a). Invasion was assessed by transwell assay in both cell lines after treatment with ONC201 for 6 h (b). Migration was measured by wound healing assay after treatment with ONC201 for 48 h (c). ONC201 significantly reduced the ability of adhesion, migration and invasion in both cell lines. Cell lysates from ECC-1 and KLE were subjected to Western blotting analysis for VEGF, Slug and E-cadherin after 24 h of treatment with ONC201 (d). ONC201 reduced the expression of VEGF and Slug and increased E-cadherin expression in both cell lines. The levels of α-tubulin served as protein loading controls. Results shown are representative of four independent experiment. *p < 0.05 and **p < 0.01