Fig. 2

Disrupting USP16 impairs PCa cell viability. a The knockdown efficiency of shRNA was confirmed by Western blot; β-actin was used as a loading control. b and c The viability of PC3 and DU145 cells was measured by CCK-8 assay at the indicated times. d and e Colony formation assays were performed in PC3 and DU145 cells transfected with the indicated shRNAs and the colony numbers of each group are shown. f PC3 cells were infected with control or USP16 (shUSP16#1-resistant) lentivirus as indicated with or without USP16 knockdown. USP16 protein level was measured by Western blot. g Relative cell growth was determined using a CCK-8 assay at day 6. Each value represents the mean ± standard deviation of three independent experiments