Fig. 3
Knockdown of USP16 impeded PCa tumour growth in vivo. a PC3-shCON and PC3-shUSP16 cells (5 × 105) were suspended in Matrigel (volume, 1:1) and subcutaneously implanted into nude mice (n = 9). Mice were sacrificed 8 weeks later, and the volumes of the xenograft tumours were determined (scale bar: 10 mm). b The weights of the xenograft tumours are shown. Error bars represent mean ± standard deviation (Mann–Whitney test; n = 9). c Kaplan–Meier analysis of tumour onset (log rank). d USP16 expression is shown as an IHC score (Mann–Whitney test). e Ki67 expression is expressed as the number of positive cells. f Haematoxylin-eosin (HE) staining and USP16 and Ki67 IHC staining in tumour xenografts (scale bar: 100 μm)