Fig. 4

USP16 regulates c-Myc stability at the post-transcriptional level. a and b Expression of USP16 and c-Myc proteins were measured by Western blot, and mRNA levels of USP16 and c-Myc were detected by qRT-PCR. c and d PC3 and DU145 cells were treated with 10 μmol/L of MG132 for 6 h and then lysed. e and f PC3 cells were transfected, treated with 50 μg/ml of CHX, and harvested at the indicated times. The plot illustrates c-Myc band intensities quantified by densitometry