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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: A positive feedback loop between TAZ and miR-942-3p modulates proliferation, angiogenesis, epithelial-mesenchymal transition process, glycometabolism and ROS homeostasis in human bladder cancer

Fig. 3

TAZ-TEAD regulates the level of miR-942-3p by binding to its promoter. a. TAZ-related miRNAs in T24 cells were identified by RNA sequencing. A heatmap of these miRNAs was generated (N.C. vs shTAZ cells, each sample was mixed with three replicates). b-c qRT-PCR detection of miR-942-3p in TAZ-knockdown or TAZ-overexpressing cells. d-e Correlation between TAZ and miR-942-3p in bladder cancer in cohort 1 and the TCGA database. f-g The Hippo signaling pathway induces miR-942-3p expression. MiR-942-3p levels in LATS1/2-deficient or TEAD2-deficient cells were determined by qRT-PCR. h. Schematic illustration of the miR-942 promoter. Predicted TEAD binding site in the miR-942 promoter (indicated by the red arrow). The section of the promoter cloned for a luciferase reporter assay and the primers used for qRT-PCR validation in a ChIP assay are indicated. j. Chromatin immunoprecipitation followed by qRT-PCR using specific primers for different regions of the miR-942 promoter in 293 T cells demonstrated that TAZ could bind to the miR-942 promoter. k. The predicted TEAD binding site was mutated, and a luciferase reporter assay was performed to validate the binding site of TAZ-TEADs in the miR-942 promoter. Data are presented as the mean ± SD of three independent experiments. *P < 0.05 and **P < 0.01 vs. the control group

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