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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: A positive feedback loop between TAZ and miR-942-3p modulates proliferation, angiogenesis, epithelial-mesenchymal transition process, glycometabolism and ROS homeostasis in human bladder cancer

Fig. 4

MiR-942-3p is involved in the proliferation, angiogenesis, migration, invasion, EMT process and redox balance of bladder cancer cells. a. MiR-433-3p was detected in bladder cancer tissue and adjacent normal tissue (cohort 1, n = 20). b. TCGA database analysis indicated the upregulation of miR-942-3p expression in bladder cancer. c. qRT-PCR was performed to determine miR-942-3p levels in SV-HUC-1 cells and bladder cancer cell lines. d. Cell viability was measured to evaluate the biological effects of miR-942-3p. e. The effect of miR-942-3p on colony formation was evaluated with a colony formation assay. f. HUVECs were cultured with conditioned medium to assess angiogenesis mediated by miR-942-3p. g. A wound healing assay was performed to evaluate cell migration. h. MiR-942-3p influenced cell migration and invasion, as determined by Transwell migration and Matrigel invasion assays, respectively. i-j The protein and mRNA expression levels of EMT markers in T24 and EJ cells transfected with pre-miR-942 or a miR-942-3p sponge were detected by western blotting and qRT-PCR, respectively. k. ROS content was evaluated by flow cytometry in different cells. Data are presented as the mean ± SD of three independent experiments. *P < 0.05 and **P < 0.01 vs. the control group

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