Fig. 5

Validation of GAS1 and LATS2 as direct targets of miR-942-3p. a. MiR-942-3p target genes predicted by searching the TargetScan, miRDB, miRWalk and miRTarBase databases. b. The potential miR-942-3p binding sites in the 3′-UTRs of GAS1 and LATS2 mRNA transcripts are shown in the schematic illustration. Lowercase letters indicate the mutated binding sites in the same 3′-UTR. c. Luciferase reporter assay analysis of 293 T cells transfected with the indicated plasmids and miR-942-3p mimics (100 nM). d. TCGA database analysis indicated negative correlations among miR-942-3p, GAS1 and LATS2. e. The miR-942-3p level was negatively correlated with the protein levels of GAS1 and LATS2. Data are presented as the mean ± SD of three independent experiments. *P < 0.05 and **P < 0.01 vs. the control group