Fig. 1
High NELFE expression predicts worse prognosis of patients with GC. a Representative IHC images of NELFE in adjacent normal tissues, primary tumor tissues and metastatic tumor tissues were shown. Magnification: 40× (upper) and 200× (bottom). b Statistical difference of NELFE protein expression among different sample types were analyzed using the Chi-square test. The samples with an IHC score ≤6 were defined as weak, and > 6 were defined as strong. c mRNA levels of NELFE in 28 paired tumor tissues and adjacent normal tissues were determined by qRT-PCR analysis, relative expression levels were represented as -ΔCT values (left), and fold changes in tumor tissues relative to adjacent normal tissues were calculated to generate the pie chart (right). d Published NELFE gene expression data were obtained from the online GEO database at https://www.ncbi.nlm.nih.gov/geo/ (GEO accession number: GSE19826 and GSE13911). e Kaplan–Meier curves for overall survival and first progression survival analysis of NELFE in GC were generated using the Kaplan–Meier plotter database (http://kmplot.com/analysis). f Representative images of NELFE immunofluorescence staining (red) in MGC-803 cells. Magnification: 40×. g NELFE protein expression in different human GC cell lines by western blotting