Fig. 3

Positive effects of NELFE on GC cell migration in vitro and metastatis in vivo. a-b Transwell migration assays were carried out at 24 h after transfection of NELFE-expressing plasmids (a) or siRNAs (b), and representative images (upper) and corresponding statistical analysis of migratory cell number (bottom) were shown. Magnification: 100×. c-d A pulmonary metastasis model of nude mice was generated using cells with stable overexpression or knockdown of NELFE (n=6 per group). Representative lung tissues (left), HE staining images of the lung tissues (middle) and statistical analysis of the number of metastatic nodules on lung surface per mouse (right). *P < 0.05, **P < 0.01