Fig. 4

CSNK2B mediates the cancer-promoting functions of NELFE in GC. a Data from Coexpedia database (http://www.coexpedia.org/) was analyzed by informatics method to identify co-expressing genes of NELFE in GC, which were ranked by sum of their edges’ log-likelyhood scores. b RNA-sequencing data from TCGA database were analyzed using the online GEPIA tool (http://gepia.cancer-pku.cn/) to validate the results obtained from Coexpedia database, and CSNK2B and BAG6 were considered as candidate genes using the following criteria: Pearson correlation coefficient ≥0.7 and P value < 0.05. c CSNK2B and BAG6 mRNA levels in AGS cells with stable knockdown of NELFE were analyzed using qRT-PCR analysis. d Western blot analyses confirming the regulation of CSNK2B protein expression by NELFE in GC cells were shown. e After transfection with siRNAs against CSNK2B, cells were lysed and western blot analysis was performed to validate the knockdown efficacies. f-h CCK-8 assays (f), colony formation assays (g) and transwell migration assays (h) were carried out at 24 h after transfection of siNC or siCSNK2B-2 into MGC-803 cells with stable NELFE overexpression or the control cells, respectively. **P < 0.01, n.s. not statistically significant