Fig. 5

NELFE promotes CSNK2B expression via activating Wnt/β-catenin signaling pathway. a At 24 h after transfection of pNELFE into MGC-803 cells, Cignal Finder 10-Pathway Reporter Array was employed to uncover potential downstream signaling pathways regulated by NELFE. b The indicated cells were transiently co-transfected with the TCF/LEF1 firefly luciferase reporter construct and the Renilla luciferase vector for 24 h, and dual-luciferase reporter assays were conducted to detect relative TCF/LFE1 luciferase activity. c-d β-catenin (c) protein and mRNA (d) expression levels in the indicated GC cell lines were determined by western blot and qPCR analyses, respectively. e CSNK2B-interacting proteins were obtained from the STRING database at http://string-db.org, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was performed by R programming language v3.5.2. f Schematic representation of two predicted TCF/LEF-binding sites in the promoter region of CSNK2B. g MGC-803 and AGS cells were transfected with CSNK2B promoter-driven luciferase constructs, 5 μM of Wnt agonist 1 was added into the cells concurrently, and CSNK2B promoter activity was measured using dual-luciferase assays after 24 h. h AGS cells with stable knockdown of NELFE and the control cells were treated with 5 μM of Wnt agonist 1, and DMSO were used as the control group. Then dual-luciferase reporter assays were performed to compare the difference of CSNK2B promoter activity between the two groups. **P < 0.01, n.s. not statistically significant