Fig. 1

Upregulation of CircZFR in cervical cancer tissues. a. CircRNAs expression profiling. Heatmap of upregulated circRNAs profiles in cervical cancer tissues (T) vs. paired adjacent non-cancer tissues (N), according to our LCHI’s cohort and GSE102686 data from the GEO database. CircZFR (hsa_circ_0072088, hsa_circRNA_103809) is significantly upregulated for cervical cancer in both cohorts. LCHI: Liaoning Cancer Hospital & Institute, FC: fold change b. Venn diagram. c. The genomic loci of ZFR gene and Sanger sequencing for the PCR products using divergent circZFR primers confirmed the head-to-tail splicing (Exon 13 and 17). d. The level of circZFR was significantly higher in cervical cancer (CC) than adjacent normal (AN) and normal cervix (N), as determined by qRT-PCR. e. The differential expression of circZFR in most cervical cancer (CC) was higher than adjacent normal tissues (AN) (86.7%, 26/30). f. qRT-PCR for the abundance of circZFR in 30 patients with cervical cancer and adjacent normal. g. The diagnostic value of circZFR for cervical cancer was evaluated by receiver operating characteristic (ROC) analysis. ***P< 0.001. AUC: area under the curve, CI: confidence interval