Fig. 2

CircZFR promoted proliferation, migration, and invasion of cervical cancer cells. a. qRT-PCR analysis of circZFR expression in HeLa and SiHa cells stably expressing circZFR or control-circZFR (circ-Ctrl). b-c. Upregulation of circZFR by stable expression of circZFR increases the proliferation ability of HeLa and SiHa cells as measured by CCK-8 assay and EdU assays (Scale bar = 200 μm). d. The colony formation assay shows that stable overexpression of circZFR increased the clonogenic ability of HeLa and SiHa cells. e-g. The wound healing and transwell matrigel migration and invasion assays show that stable overexpression of circZFR enhanced cell migration and invasion of HeLa and SiHa cells (Scale bar in e = 200 μm, scale bar in f and g = 50 μm). h. Significant decrease in the number of cells in the G0-G1 phase of the cell cycle and increased cells in the S phase after overexpression of circZFR as detected by Flow Cytometry (FCM). *P< 0.05, **P< 0.01, ***P< 0.001