Fig. 4

CircZFR activation of E2F1 pathway promoted the progression of cervical cancer. a. Heatmap showed the genes altered (Fold change > 2, P < 0.05) in cervical cancer compared with normal tissues according to TCGA and GTEx databases. Red refers to higher, and green refers to lower expression. b. Genes enrichment was performed using Cytoscape/BINGO software. A total of 14 upregulated genes in cervical cancer were enriched in the biological process of DNA replication, cell cycle progress, and cell proliferation. c. Relative mRNA expression of 14 candidate genes was detected by qRT-PCR in HeLa and SiHa cells stably overexpressing circZFR or circ-Ctrl. The mRNA expression of CCNB1, CCNA2, CDC25A, CDC6, and TFDP1 was increased after the overexpression of circZFR. d. E2F1, E2F4 and EGR1 were identified as the transcription factors (TFs) of CCNB1, CCNA2, CDC25A, CDC6, and TFDP1 using ChIPbase. *P< 0.05, **P< 0.01, ***P< 0.001