Fig. 2

ACTL6A enhances CDK2 transcriptional activity by increasing the enrichment of MYC and KAT5 on its promoters. a A Venn diagram among MYC related genes, ACTL6A related genes, and cell cycle related genes. b Real-time PCR analysis of the expression of 9 genes in SUM159PT cells with altered ACTL6A expression. c Western blot analysis of CDK2 in indicated cells. d Luciferase activity of CDK2 reporter were detected in the indicated cells. e Schematic illustration of the PCR fragments of the human CDK2 gene promoters (upper panel). Chromatin immunoprecipitation (ChIP) assays were performed in SUM159PT-ACTL6A-Flag cells using antibodies against Flag and MYC to identify the occupancy on CDK2 gene promoters. Immunoglobulin G (IgG) was used as a negative control. f Enrichment of MYC on p2 fragment of CDK2 promoter. g IP assay revealed that ACTL6A formed complex with MYC and KAT5 in SUM159PT-ACTL6A cells. h Silencing of ACTL6A abrogated interaction between MYC and KAT5. (I) Enrichment of KAT5 on p2 fragment of CDK2 promoter. j Appearance of acetylated histone H3K14ac and histone H4 (K12ac, K16ac, K5ac and K8ac) on p2 fragment of CDK2 promote in the indicated cells. Each bar represents the mean ± S.D. of three independent experiments. Two-tailed Student’s t test was used. *P < 0.05, **P < 0.01, ***P < 0.001 and n.s. for no significance