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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: LINC00460/DHX9/IGF2BP2 complex promotes colorectal cancer proliferation and metastasis by mediating HMGA1 mRNA stability depending on m6A modification

Fig. 5

LINC00460 mediated regulation of HMGA1 mRNA stability. a, b Effect of LINC00460 KD or OE on the expression of IGF2BP2, DHX9 and HMGA1 protein level in HCT116 and SW480 cells, as assessed by Western blot. c, d, e, f Relative mRNA expression levels of LINC00460, IGF2BP2, DHX9 and HMGA1 in HCT116 and SW480 cells ± LINC00460 KD/OE (*p < 0.05, **p < 0.01, ***p < 0.001). g, h Western blot assay was performed to measure DHX9, IFG2BP2 and HMGA1 protein level after transfection of DHX9/IGF2BP2 siRNA, control siRNA in LINC00460 overexpressed CRC cells. i, j, k, l RIP assay shows interaction declined between HMGA1 and DHX9/IGF2BP2 after knockdown of LINC00460 in CRC cells

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