Fig. 2

Enforced expression of ESCO2 promotes LUAD cell growth, metastasis, and colony formation in vitro. a–c ESCO2-FLAG constructs were transfected into NCI-H1975 and A549 cells; FLAG was immunostained using anti-FLAG antibodies (a); ESCO2 mRNA and protein expression levels were detected by q-PCR (b) and western blotting (c), respectively. d GSEA showed that the cell cycle signatures and DNA replication signatures had a significant positive correlation with the ESCO2 mRNA expression levels in TCGA LUAD cohort. e–g The effects of ESCO2 overexpression on cell growth (e), migration and invasion (f), and colony formation (g) were detected in NCI-H1975 and A549 cells. Data are the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001