Table 1 Primary antibodies for western blot
Antibody | Concentration | Specificity | Company |
|---|---|---|---|
HOXA10 | 1:1000 | Mouse | Santa Cruz |
E-cadherin | 1:1000 | Rabbit | Cell Signaling Technology |
N-cadherin | 1:1000 | Rabbit | Cell Signaling Technology |
Vimentin | 1:1000 | Rabbit | Cell Signaling Technology |
Snail | 1:1000 | Rabbit | Cell Signaling Technology |
Slug | 1:1000 | Rabbit | Cell Signaling Technology |
TGFB1 | 1:2000 | Rabbit | Proteintech |
TGFB2 | 1:1000 | Mouse | Abcam |
TGFB3 | 1:500 | Rabbit | Proteintech |
GAPDH | 1:5000 | Mouse | Proteintech |
Smad2 | 1:1000 | Rabbit | Cell Signaling Technology |
P-Smad2 | 1:1000 | Rabbit | Cell Signaling Technology |
Smad3 | 1:1000 | Rabbit | Cell Signaling Technology |
P-Smad3 | 1:1000 | Rabbit | Cell Signaling Technology |
Smad2/3 | 1:1000 | Rabbit | Cell Signaling Technology |
Histone H3 | 1:2000 | Rabbit | Cell Signaling Technology |
P-Smad2/3 | 1:1000 | Rabbit | Cell Signaling Technology |
METTL3 | 1:1000 | Rabbit | Abcam |