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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Long non-coding RNA CCDC183-AS1 acts AS a miR-589-5p sponge to promote the progression of hepatocellular carcinoma through regulating SKP1 expression

Fig. 2

CCDC183-AS1 promoted HCC cell proliferation and metastasis in vitro and in vivo. a-b. CCK-8 assays were used to evaluate HCC cells proliferation after CCDC183-AS1 overexpression (a) or knockdown (b). c-d. Transwell experiments were performed to analyze the cell migration and invasion in CCDC183-AS1 overexpressing (c) and knockdown (d) HCC cells. e. Image of subcutaneous tumor tissues in CCDC183-AS1-overexpressing group and control group. f-g. The volume (f) and weight (g) of subcutaneous tumor tissues were measured. h. Relative expression levels of Ki67 were observed in subcutaneous tumor tissues by IHC. i. In orthotopic model mice, representative images of the liver of CCDC183-AS1 overexpressing group and control group. j. The size of the largest visible liver nodule was measured. k. Quantifications of visible surface liver metastatic nodules. l. Representative images in hematoxylin-eosin (HE) staining of the liver metastasis of 97H cells. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001; ns, no significance

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