Fig. 3

CCDC183-AS1 acted as a ceRNA to sponge miR-589-5p in HCC cells. a. Bioinformatic prediction (http://carolina.imis.athenainnovation.gr/diana_tools/web/index.php?r=lncbasev2%2Findex-predicted) of the potential binding site of miR-589-5p and CCDC183-AS1. b. Relative expression levels of miR-589-5p were evaluated by qRT-PCR in HLF cells transfected with the miR-589-5p mimics or inhibitor, respectively. c-d. The relative luciferase activities were detected in HLF cells after co-transfection with CCDC183-AS1-WT or CCDC183-AS1-MUT and mimics, inhibitor or NC, respectively. e-f. Anti-Ago2 RIP assay was executed in HLF cells after transfection with miR-589-5p mimics and NC, followed by qRT-PCR to detect CCDC183-AS1. g-h. qRT-PCR analysis of miR-589-5p expression in HCC cells after CCDC183-AS1 overexpression (g) or knockdown (h). Data are presented as mean ± SD. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001; ns, no significance