Fig. 5

SKP1 is a direct target of miR-589-5p. a. Schematic diagram of miR-589-5p putative binding site in the WT and MUT 3’UTR of SKP1. b. The relative luciferase activities were detected in HLF and 97H cells after co-transfection with SKP1–3’UTR-WT or SKP1–3’UTR-MUT and mimics or NC, respectively. c-d. Relative mRNA (c) and protein (d) levels of SKP1 were evaluated by qRT-PCR and Western blot in HLF and 97H cells transfected with the miR-589-5p mimics or inhibitor, respectively. e. Correlation between miR-589-5p and SKP1 expression in paired HCC tissues (n=44). f. SKP1 protein levels in HCC cells after CCDC183-AS1 overexpression or knockdown. Data are presented as mean ± SD. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001; ns, no significance