Fig. 6

SKP1 promoted HCC cell proliferation and metastasis. a. The overexpression and knockdown efficiency of SKP1 were confirmed by Western blot. b-c. CCK-8 assays were used to evaluate HLF cells proliferation after SKP1 overexpression (b) or knockdown (c). d-e. Cellular migratory and invasive capabilities were assessed by transwell assays in HLF cells after SKP1 overexpression (d) or knockdown (e). f. Western blot analysis to determine expression level of the cell cycle and EMT relative marker in SKP1 overexpressed and knockdown HLF cells. g. The expression levels of SKP1 in HCC and adjacent non-cancer tissues were evaluated by Western blot(n=80). h-i. Statistical analysis of relative SKP1 levels in HCC tissues compared to normal tissue controls (n=80). Data are presented as mean ± SD. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001; ns, no significance. N, normal tissue; T, tumor tissue