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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Splicing factors control triple-negative breast cancer cell mitosis through SUN2 interaction and sororin intron retention

Fig. 3

Effect of candidate splicing factor knockdown on sister chromatid cohesion. a Effect of splicing factor knockdown on nuclear phenotype in Hs578T cells 72 h after knockdown. Scale bar = 50 μm. b Percentage of cells displaying an abnormal nuclear phenotype 72 h after knockdown. Mean + stdev of at least 3 positions. c Percentage of p-Histone H3 positive cells upon splicing factor knockdown 72 h after knockdown. Nocodazole treatment was used as a positive control. Mean + stdev of 3 biological replicates. d Nuclear phenotype of p-histone H3 positive cells upon SNRPD2 knockdown in MDA-MB-231. Some cells with irregular nuclear phenotype are p-histone H3 positive (blue squares), while some are negative (white squares). Scale bar = 50 μm. e Overview of the factors involved in sister chromatid cohesion. Adapted from Peters et al, 2012. f Effect of SNRPD2, SNRPD3 and NHP2L1 knockdown on RNA expression levels of sororin, ESPL1, MAU2 and SMC1 in Hs578T 72 h after knockdown. Mean + stdev of 3 biological replicates. g Effect of SNRPD2, SNRPD3 and NHP2L1 knockdown on sororin, ESPL1, MAU2 and SMC1 expression levels 1, 2 and 3 days after knockdown in Hs578T. Mean + stdev of three biological replicates. (H) Protein MAU2, SMC1 and sororin levels 3 days after knockdown of SNRPD2, SNRPD3 and NHP2L1. Statistical significance was determined using ANOVA correcting for multiple testing. * p < 0.05, ** p < 0.01, *** p < 0.001

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