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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Splicing factors control triple-negative breast cancer cell mitosis through SUN2 interaction and sororin intron retention

Fig. 5

SNRPD2, SNRPD3 and NHP2L1 protein interactions. a Characterization of Hs578T GFP BAC-reporter cell lines for splicing factors SNRPD2, SNRPD3 and NHP2L1. Images were captured 72 h after transfection. Scale bar = 30 μm. b Overlap of proteins residing in SNRPD2, SNRPD3 and NHP2L1 complexes, respectively. Splicing factors belonging to the nine candidates selected from the primary screen are labeled in purple. Orange: proteins that were selected for further validation. c Nuclear phenotype 72 h after SUN2 knockdown in Hs578T and MDA-MB-231 cells. Scale bar = 30 μm. d Co-immunoprecipitation of SNRPD2, SNRPD3 and NHP2L1 Hs578T BAC GFP-reporters with SUN2. e Alternative splicing of sororin intron 1 upon SUN2 knockdown. Experiments were performed in biological triplicates. Significance was determined using a Student’s T-test. * p < 0.05, ** p < 0.01

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