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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinoma

Fig. 1

ITPR3 was commonly overexpressed in BCa cells and tissues. a Representative pictures of ITPR3 protein expression of different pathologic T stage in bladder cancer tissue chips detected by IHC. b Quantification of the ITPR3 protein in bladder cancer and normal peritumor tissues. Scale bar = 100 μm. c western blot analysis of ITPR3 expression levels in human bladder cancer cell lines and the normal bladder cell line SV-HUC-1. β-actin was used as the loading control. d Quantitative real-time PCR (qRT-PCR) analysis of the mRNA expression levels of ITPR3 in bladder cancer cell lines and the normal bladder cell line SV-HUC-1. 18S was applied as the endogenous control. Data are presented as the mean ± SEM, n = 3. e ITPR3 protein expression level was analyzed in eleven cases of fresh human bladder cancer tissues and corresponding normal tissues by western blot assay. β-actin was used as internal loading control *p < 0.05; **p < 0.01; ***p < 0.001. NAT: normal tissue adjacent to the tumor; Ta-T4: Pathologic T stage Ta, T1–4; NMIBC: non-muscle invasive bladder cancer; MIBC; muscle invasive bladder cancer

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