Fig. 4From: ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinomaITPR3 loss inhibits the migration and invasiveness of bladder cancer cells in vitro. Representative images of wound healing in 5637 (a) and 253 J (b) shITPR3 or shCon cells. Quantitative data are shown on the right. b Cell migration and invasion ability was assessed by the Transwell assay with or without Matrigel in 5637 (c) and 253 J (d) shITPR3 or shCon cells. Quantification analysis is shown below. MMP2 and MMP9 expression was detected by a western blot assay in 5637 (e) and 253 J (f) shITPR3 or shCon cells. The bar chart represents the ratios of MMP2 and MMP9 relative to β-actin (as a loading control) and is shown on the right. **p < 0.01; ***p < 0.001Back to article page