Fig. 5

ITPR3 inhibition blocks epithelial-mesenchymal transition in bladder cancer cells. a The mesenchymal markers N-cadherin and vimentin, the epithelial marker E-cadherin, and the transcription factors snail 1 and snail 2 were detected by western blotting after ITPR3 knockdown in 5637 and 253 J cells. β-actin served as an internal control. b The expression of E-cadherin, N-cadherin, Vimentin, Snail 1, and Snail 2 was detected by western blotting after treatment with TGF-β (10 ng/mL) in 5637 and 253 J cells transfected with ITPR3 shRNA or shCon. β-actin served as an internal control. The results of immunofluorescence assays for E-cadherin and N-cadherin expression were also determined in 5637 (c) and 253 J (d) cells transfected with ITPR3 shRNA or shCon. Scale bars, 100 μm