Fig. 8

ITPR3 regulates proliferation and stemness via the NF-ĸB/CD44 signaling pathway in BCa. a An MTT assay was conducted to evaluate the viability of 5637 and 253 J shITRR3 or shCon cells after treatment with TNFα or overexpression of CD44. b An EdU staining assay was conducted to assess the proliferation ability of 5637 and 253 J shITRR3 or shCon cells treated as described above. c Colony formation assays were also performed in 5637 and 253 J shITRR3 or shCon cells treated as described above. Quantification analysis is shown below. d The cancer stemness was evaluated by a tumorsphere formation assay in 5637 and 253 J shITRR3 or shCon cells treated as described above. The scale bar represents 100 μm. Quantification analysis is shown below. ^*p < 0.05, ^**p < 0.01; ^***p < 0.001 versus the scrambled shRNA (shCon) group, ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 versus the ITPR3 shRNA group. CD44 OE: CD44 overexpression. shCon: Control shRNA