Fig. 2

HOXA10 promoted the expression of HDAC1 in LAD cells. a Expression of HDAC1 in LAD tissues (n = 42) and paracancerous tissues (n = 42) by RT-qPCR. b Immunohistochemical detection of HDAC1 in LAD tissues and paracancerous tissues (200×). c Pearson correlation of HOXA10 and HDAC1 expression in LAD. d HDAC1 mRNA expression in HBE cells and 5 LAD cell lines by RT-qPCR, normalized to GAPDH. e HDAC1 protein expression in HBE cells and 5 LAD cell lines by Western blot analysis, normalized to GAPDH. f HOXA10 and HDAC1 protein expression in A549 cells after HOXA10 silencing by Western blot analysis normalized to GAPDH. g HOXA10 binding to HDAC1 promoter by ChIP. h Dual luciferase reporter gene assay to verify the binding between HOXA10 and HDAC1. * p < 0.05 vs. the paracancerous tissues (panels a and b), HBE cells (panels d and e), A549 cells transfected with sh-NC (panel f), DNA-protein complex fragments incubated with IgG (panels g), or cells transfected with sh-NC and HDAC1-wt (panel h). All experiments were done in triplicate