Fig. 3

HDAC1 is the mediator of HOXA10 in LAD cells to regulate proliferation, apoptosis, invasion and migration. a HDAC1 protein expression following transfection with oe-NC or oe-HDAC1 by Western blot analysis, normalized to GAPDH. A549 cells were transfected with sh-NC + oe-NC, sh-HOXA10 + oe-NC or sh-HOXA10 + oe-HDAC1 for panels B-G. b HOXA10 and HDAC1 protein expression by Western blot analysis, normalized to GAPDH. c Cell viability detection by CCK-8. d The detection of cell apoptosis by flow cytometry. e The cell cycle distribution by flow cytometry. f Cell migration by scratch test. g Cell invasion by Transwell assay. All experiments were done in triplicate. * p < 0.05