Fig. 4

HDAC1 promoted DNMT1 deacetylation. a Expression of DNMT1 in LAD tissues (n = 42) and paracancerous tissues (n = 42) by RT-qPCR, normalized to GAPDH. b Immunohistochemical detection of DNMT1 in LAD tissues (n = 42) and paracancerous tissues (n = 42) (200 ×). c DNMT1 expression in HBE cells and 5 LAD cell lines by Western blot analysis, normalized to GAPDH. d Silencing efficiency of HDAC1 by Western blot analysis, normalized to GAPDH. e The expression of HDAC1 and DNMT1 when HDAC1 was silenced by Western blot analysis, normalized to GAPDH. f Co-IP analysis of the interaction between HDAC1 and DNMT1 relative to IgG. g In vitro acetylation of DNMT1. h In vivo ubiquitination of DNMT1 relative to GAPDH. i Pulse tracking analysis for the effect of HDAC1 on the stability of DNMT1. All experiments were done in triplicate. * p < 0.05 vs. the paracancerous tissues (panels a and b), HBE cells (panel c) or cell treated with sh-NC (panels d, e, and i)