Fig. 5

HDAC1 silencing downregulated the expression of DNMT1 to inhibit the proliferation of LAD cells. A549 cells were transfected with sh-NC + oe-NC, sh-HDAC1 + oe-NC or sh-HDAC1 + oe-DNMT1. a The expression of HDAC1 or DNMT1 by Western blot analysis, normalized to GAPDH. b CCK-8 test to detect cell viability. c Flow cytometry to detect cell apoptosis. d Flow cytometry to detect cell cycle distribution. e Scratch test to detect cell migration ability. f Transwell assay to detect cell invasion. All experiments were done in triplicate. * p < 0.05