Fig. 7

DNMT1-induced downregulation of KLF4 promoted the proliferation, migration, and invasion of LAD cells. a Western blot analysis to screen out the sequence with best silencing effects on KLF4 (normalized to GAPDH) following transfection with sh-NC, sh-KLF4–1, sh-KLF4–2, and sh-KLF4–3. A549 cells were transfected with sh-NC + sh-NC, sh-DNMT1 + sh-NC or sh-DNMT1 + sh-KLF4 for panels b-g. b The protein expression of DNMT1 or KLF4 by Western blot analysis, normalized to GAPDH. c CCK-8 test to detect cell viability. d Flow cytometry to detect cell apoptosis. e Flow cytometry to detect cell cycle distribution. f Scratch test to detect cell migration ability. g Transwell assay to detect cell invasion. All experiments were done in triplicate. * p < 0.05 vs. the sh-NC + sh-NC group (A549 cells transfected with sh-NC + sh-NC). # p < 0.05 vs. the sh-DNMT1 + sh-NC group (A549 cells transfected with sh-DNMT1 + sh-NC)