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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Long non-coding RNA MAPKAPK5-AS1/PLAGL2/HIF-1α signaling loop promotes hepatocellular carcinoma progression

Fig. 5

MAPKAPK5-AS1 acts as a sponge of miR-154-5p in HCC cells. a Subcellular fractionation assay was applied to determine MAPKAPK5-AS1 subcellular localization. b 15 putative targets of MAPKAPK5-AS1 was predicted by applying two bioinformatics prediction tools. c qRT-PCR showed that MAPKAPK5-AS1 negatively regulated miR-154-5p expression in HCC cells. d The predicted binding sites between miR-154-5p and MAPKAPK5-AS1 and mutant sequences of the potential miR-154-5p binding site in MAPKAPK5-AS1. Additionally, the result of luciferase reporter assay is shown here. (E) Anti-Ago2 RIP assay was performed in HCCLM3 with miR-154-5p overexpression, followed by qRT-PCR to detect the level of MAPKAPK5-AS1 or miR-154-5p associated with Ago2. f The expression level of miR-154-5p in HCC tissues and adjacent non-tumor tissues. g The relativity between MAPKAPK5-AS1 and miR-154-5p in HCC tissues. *p < 0.05

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